Team:SDU-Denmark/labnotes2

From 2010.igem.org

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== Group: Photosensor ==
== Group: Photosensor ==
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=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 from transformation 20/7 ===
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=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 K081005] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 R0011] in pSB1A2 from transformation 20/7 ===
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Start date: 22/07    End date: 22/07<br>
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Start date: July 22nd<br>
''Methods:''  Miniprep, gel electrophoresis and nano drop <br><br>
''Methods:''  Miniprep, gel electrophoresis and nano drop <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2] <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2] <br><br>
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''Analysis:''<br>
''Analysis:''<br>
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Sample 1-4 of BBa_K081005 were pooled and frozen and sample 1-4 of BBa_R0011 were too.<br>
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Sample 1-4 of K081005 were pooled and frozen and sample 1-4 of R0011 were too.<br>
<br>
<br>
=== Gel extraction test (GFX vs. Fermentas) ===
=== Gel extraction test (GFX vs. Fermentas) ===
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Start date: 25/07    End date: 25/07<br>
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Start date: July 25th <br>
''Methods:'' Gel electrophoresis, gel extraction and nano drop <br><br>
''Methods:'' Gel electrophoresis, gel extraction and nano drop <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.2 DE1.2][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3] <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.2 DE1.2][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3] <br><br>
''Experiment done by:''  Maria<br><br>
''Experiment done by:''  Maria<br><br>
''Notes:'' <br><br>
''Notes:'' <br><br>
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FlhD/C tq PCR product (5 green) is used in the test.<br>
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FlhD/C taq PCR product (5 green) is used in the test.<br>
PCR product is diluted in H2O to reach a sample concentration below 100ng/uL.<br>
PCR product is diluted in H2O to reach a sample concentration below 100ng/uL.<br>
Nanodrop:<br>
Nanodrop:<br>
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</tr>
</tr>
</table><br>
</table><br>
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A total of 70uL is loaded onto a 2% agarose gel.<br>
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A total of 70 microlitres is loaded onto a 2% agarose gel.<br>
For the GFX gel extraction 350mg of gel was used.<br>
For the GFX gel extraction 350mg of gel was used.<br>
For the Fermentas extraction 460mg of gel was used.<br>
For the Fermentas extraction 460mg of gel was used.<br>
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</table><br><br>
</table><br><br>
''Analysis:''<br>
''Analysis:''<br>
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There is extracted more DNA with the fermentas kit. This may be due to the short centrifugation time in the GFX protocol. Furthermore the GFX kit that was used was old and the buffers may have been contaminated.<br><br>
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More DNA is extracted with the fermentas kit. This may be due to the short centrifugation time in the GFX protocol. Furthermore the GFX kit that was used was old and the buffers may have been contaminated.<br><br>
== Group: Retinal ==
== Group: Retinal ==
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<br>
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=== Transformation of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 (constitutive promoter and RBS combined),[http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2, pSB3C5 w. [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] and pSB3T5 w. BBa_J04450 in Top 10 E.Coli ===
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=== Transformation of [http://partsregistry.org/Part:BBa_K081005 K081005] in pSB1A2 (constitutive promoter and RBS combined),[http://partsregistry.org/Part:BBa_R0011 R0011] in pSB1A2, pSB3C5 w. [http://partsregistry.org/Part:BBa_J04450 J04450] and pSB3T5 w. J04450 in Top 10 E.Coli ===
Start date: 19/07    End date: 20/07<br>
Start date: 19/07    End date: 20/07<br>
''Methods:''  ON culture, making competent cells, transformation <br><br>
''Methods:''  ON culture, making competent cells, transformation <br><br>
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--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br>
--[[User:Tipi|Tipi]] 08:17, 21 July 2010 (UTC)<br><br>
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=== Coloni PCR of [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 and [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 ===
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=== Coloni PCR of [http://partsregistry.org/Part:BBa_R0011 R0011] in pSB1A2 and [http://partsregistry.org/Part:BBa_K081005 K081005] in pSB1A2 ===
Start date: 20/07    End date: 20/07<br>
Start date: 20/07    End date: 20/07<br>
''Methods:''  Coloni PCR and gel electrophoresis <br><br>
''Methods:''  Coloni PCR and gel electrophoresis <br><br>
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''Experiment done by:'' Maria and LC<br><br>
''Experiment done by:'' Maria and LC<br><br>
''Notes:''<br>
''Notes:''<br>
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6 colonies transformed with pSB1A2 w. BBa_R0011 and 4 colonies transformed with pSB1A2 w. BBa_K081005 (see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br>
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6 colonies transformed with pSB1A2 w. R0011 and 4 colonies transformed with pSB1A2 w. K081005 (see [https://2010.igem.org/Team:SDU-Denmark/labnotes2#Transformation_of_K081005_in_pSB1A2_.28constitutive_promoter_and_RBS_combined.29.2CR0011_in_pSB1A2.2C_pSB3C5_w._J04450_and_pSB3T5_w._J04450_in_Top_10_E.Coli Transformation]) was used for coloni PCR. <br>
Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br>
Colonies with K081005 was denoted A1, A2 A3 and A4 respectively.<br>
Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br>
Colonies with R0011 was denotes B1, B2, B3, B4, B5 and B6 respectively<br>

Latest revision as of 19:23, 24 October 2010