Team:SDU-Denmark/labnotes2

From 2010.igem.org

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== Group: Photosensor ==
== Group: Photosensor ==
=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 from transformation 20/7 ===
=== Miniprep of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 and [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 from transformation 20/7 ===
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Start date: 22/07    End date: 22/07<br>
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Start date: July 22nd<br>
''Methods:''  Miniprep, gel electrophoresis and nano drop <br><br>
''Methods:''  Miniprep, gel electrophoresis and nano drop <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2] <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.2 MP1.2] <br><br>
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=== Gel extraction test (GFX vs. Fermentas) ===
=== Gel extraction test (GFX vs. Fermentas) ===
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Start date: 25/07    End date: 25/07<br>
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Start date: July 25th <br>
''Methods:'' Gel electrophoresis, gel extraction and nano drop <br><br>
''Methods:'' Gel electrophoresis, gel extraction and nano drop <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.2 DE1.2][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3] <br><br>
''Protocol:''[https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.2 DE1.2][https://2010.igem.org/Team:SDU-Denmark/protocols#DE1.3 DE1.3] <br><br>
''Experiment done by:''  Maria<br><br>
''Experiment done by:''  Maria<br><br>
''Notes:'' <br><br>
''Notes:'' <br><br>
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FlhD/C tq PCR product (5 green) is used in the test.<br>
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FlhD/C taq PCR product (5 green) is used in the test.<br>
PCR product is diluted in H2O to reach a sample concentration below 100ng/uL.<br>
PCR product is diluted in H2O to reach a sample concentration below 100ng/uL.<br>
Nanodrop:<br>
Nanodrop:<br>
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</tr>
</tr>
</table><br>
</table><br>
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A total of 70uL is loaded onto a 2% agarose gel.<br>
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A total of 70 microlitres is loaded onto a 2% agarose gel.<br>
For the GFX gel extraction 350mg of gel was used.<br>
For the GFX gel extraction 350mg of gel was used.<br>
For the Fermentas extraction 460mg of gel was used.<br>
For the Fermentas extraction 460mg of gel was used.<br>
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</table><br><br>
</table><br><br>
''Analysis:''<br>
''Analysis:''<br>
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There is extracted more DNA with the fermentas kit. This may be due to the short centrifugation time in the GFX protocol. Furthermore the GFX kit that was used was old and the buffers may have been contaminated.<br><br>
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More DNA is extracted with the fermentas kit. This may be due to the short centrifugation time in the GFX protocol. Furthermore the GFX kit that was used was old and the buffers may have been contaminated.<br><br>
== Group: Retinal ==
== Group: Retinal ==

Revision as of 17:01, 22 October 2010