Team:SDU-Denmark/labnotes

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(Difference between revisions)
(Miniprep and transformation of K274210 in pSB1A2)
(Restriction digest of BBa_E0040 miniprep product)
 
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''Analysis:'' The transformations succeeded. <br><br>
''Analysis:'' The transformations succeeded. <br><br>
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=== Coloni PCR of pSB1A2 w. [http://partsregistry.org/Part:BBa_E0040 BBa_E0040] (transformation 13/7) ===
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=== Coloni PCR of pSB1A2 w. [http://partsregistry.org/Part:BBa_E0040 E0040] (transformation 13/7) ===
Start date: July 14th <br>
Start date: July 14th <br>
''Methods:''  Taq coloni PCR and gel electrophoresis <br><br>
''Methods:''  Taq coloni PCR and gel electrophoresis <br><br>
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--[[User:Tipi|Tipi]] 16:09, 19 July 2010 (UTC)
--[[User:Tipi|Tipi]] 16:09, 19 July 2010 (UTC)
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=== Transformation of [http://partsregistry.org/Part:BBa_K081005 BBa_K081005] in pSB1A2 (constitutive promoter and RBS combined) and [http://partsregistry.org/Part:BBa_R0011 BBa_R0011] in pSB1A2 in Top 10 E.Coli ===
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=== Transformation of [http://partsregistry.org/Part:BBa_K081005 K081005] in pSB1A2 (constitutive promoter and RBS combined) and [http://partsregistry.org/Part:BBa_R0011 R0011] in pSB1A2 in Top 10 E.Coli ===
Start date: July 15th - 16th <br>
Start date: July 15th - 16th <br>
''Methods:''  Overnight culture, making competent cells, transformation <br><br>
''Methods:''  Overnight culture, making competent cells, transformation <br><br>
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--[[User:Lclund|Lclund]] 10:00, 19 July 2010 (UTC)
--[[User:Lclund|Lclund]] 10:00, 19 July 2010 (UTC)
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==== Restriction digest of [http://partsregistry.org/Part:BBa_E0040 BBa_E0040] miniprep product ====
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==== Restriction digest of [http://partsregistry.org/Part:BBa_E0040 E0040] miniprep product ====
Start date: July 16th<br>
Start date: July 16th<br>
''Methods:''  Restriction digest <br><br>
''Methods:''  Restriction digest <br><br>
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<br><br>
<br><br>
''Analysis:'' Sample 2 and 3 got pooled and frozen, 1 and 4 were discarded, since their length didn't seem exactly right.<br><br>
''Analysis:'' Sample 2 and 3 got pooled and frozen, 1 and 4 were discarded, since their length didn't seem exactly right.<br><br>
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Nr. 12 in the freezer = BBa_E0040 miniprep product in pSB1A2.<br>
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Nr. 12 in the freezer = E0040 miniprep product in pSB1A2.<br>
--[[User:Lclund|Lclund]] 10:00, 19 July 2010 (UTC)
--[[User:Lclund|Lclund]] 10:00, 19 July 2010 (UTC)

Latest revision as of 19:16, 24 October 2010