Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Amplification of pSB3K3 with Taq)
(Transformation of TOP10 e. coli with BBa_K274210)
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== Group: Retinal ==
== Group: Retinal ==
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=== Transformation of TOP10 ''e. coli'' with [http://partsregistry.org/Part:BBa_K274210 BBa_K274210]===
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=== Transformation of TOP10 ''e. coli'' with [http://partsregistry.org/Part:BBa_K274210 K274210]===
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Experiment continued from last week.
Experiment continued from last week.
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Gel was loaded with DNA-ladder plus, with the upper marker at 5000 bp.<br><br>
Gel was loaded with DNA-ladder plus, with the upper marker at 5000 bp.<br><br>
''Results:'' We found plasmids at the expected 5 kb marker in colonies 1-8. Successfull plates were kept in incubator over night. They will be made into overnight cultures and thereafter frozen.<br><br>
''Results:'' We found plasmids at the expected 5 kb marker in colonies 1-8. Successfull plates were kept in incubator over night. They will be made into overnight cultures and thereafter frozen.<br><br>
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''Analysis:'' It seems we have transformed our cells with BBa_K274210. Confirmation will come when we run experiments to demonstrate presence of beta-carotene. <br><br>
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''Analysis:'' It seems we have transformed our cells with K274210. Confirmation will come when we run experiments to demonstrate presence of beta-carotene. <br><br>
=== Transformation of [http://partsregistry.org/Part:BBa_R0011 BBa_R0011 ]and [http://partsregistry.org/Part:BBa_I0500 BBa_I0500] ===
=== Transformation of [http://partsregistry.org/Part:BBa_R0011 BBa_R0011 ]and [http://partsregistry.org/Part:BBa_I0500 BBa_I0500] ===

Revision as of 18:37, 24 October 2010