Team:SDU-Denmark/labnotes

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''Date'': July 13th <br><br>
''Date'': July 13th <br><br>
''Experiment done by:'' Ann and Pernille <br><br>
''Experiment done by:'' Ann and Pernille <br><br>
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''methods'': The experiment was perform in the ''E.coli'' strain MG1655. The DNA purification was done in duplicates and are saved in the freezer in tube 8 and 9. (green label). There DNA concentration was measure by Nano Drop and there contantretion are 16,87ng/uL and 19,25ng/uL, respectively. <br><br>
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''methods'': The experiment was perform in the ''E.coli'' strain MG1655. The DNA purification was done in duplicates and are saved in the freezer in tube 8 and 9 (green label). There DNA concentration was measure by Nano Drop and there contantretion are 16,87ng/uL and 19,25ng/uL, respectively. <br><br>
''protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#Genomic_DNA_purification GP1.1] and [https://2010.igem.org/Team:SDU-Denmark/protocols#Colony_PCR CP1.1]. <br><br>
''protocol'': [https://2010.igem.org/Team:SDU-Denmark/protocols#Genomic_DNA_purification GP1.1] and [https://2010.igem.org/Team:SDU-Denmark/protocols#Colony_PCR CP1.1]. <br><br>
''Notes'': The experiment was generally preformed corresponding to the protocol but small modification was made. In the DNA purification experiment we used 300uL instead of 200uL of the overnight culture and it is important to remember that the 800uL precipitation solution, containing 80uL solution and 720uL deionized water, are made directly before use. <br>
''Notes'': The experiment was generally preformed corresponding to the protocol but small modification was made. In the DNA purification experiment we used 300uL instead of 200uL of the overnight culture and it is important to remember that the 800uL precipitation solution, containing 80uL solution and 720uL deionized water, are made directly before use. <br>

Revision as of 19:27, 21 October 2010