Team:SDU-Denmark/labnotes

From 2010.igem.org

(Difference between revisions)
(Extraction of pSB3K3 plasmids with inserted RFP (Red fluorescent protein) from E. coli MG1655)
(Exp. 3)
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''Methods:'' MiniPrep, NanoDrop and gel electrophoresis. <br><br>
''Methods:'' MiniPrep, NanoDrop and gel electrophoresis. <br><br>
''protocols:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.1 MP1.1] <br><br>
''protocols:'' [https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.1 MP1.1] <br><br>
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''Notes:'' We used 10 ml af a culture in exponential phase (1ml cells from an ON culture + 9ml LB medium, incubated at 37 degrees celcius for 4 hours)and Fermentas GeneJET Plasmid Miniprep kit. We mixed 4 microlitres of sample and 4 microlitres 1x agarose loading dye and loaded on a 1,5% agarose gel,used Generuler DNA ladder mix (100-10000 nucleotides)from Fermentas as marker. <br><br>
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''Notes:'' We used 10 ml af a culture in exponential phase (1ml cells from an ON culture + 9ml LB medium, incubated at 37 degrees celcius for 4 hours) and Fermentas GeneJET Plasmid Miniprep kit. We mixed 4 microlitres of sample and 4 microlitres 1x agarose loading dye and loaded on a 1,5% agarose gel,used Generuler DNA ladder mix (100-10000 nucleotides)from Fermentas as marker. <br><br>
''Results:'' Nanodrop: Tube 1: 38.7 ng/ul Tube 2: 32.4 ng/ul. <br><br>
''Results:'' Nanodrop: Tube 1: 38.7 ng/ul Tube 2: 32.4 ng/ul. <br><br>
Gel electrophoresis: Bands were detected.<br><br>
Gel electrophoresis: Bands were detected.<br><br>

Revision as of 18:02, 21 October 2010