Team:SDU-Denmark/K343007

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[[Image:Team SDU-Denmark OD WT+PS.JPG|thumb|center|400px|Growth curve showing measured OD at 550nm. Samples were taken every hour over a 12 hour perion + one sample after 24 hours. In graph ''E. coli'' strain MG1655-pSB3T5-k343007 and MG1655-pSB1C3-K343007 is compaired with Wild type ''E. coli'' strain MG1655 showing no significant difference between the three graphs. No Lag phase is seen. All data can be seen under [http://2010.igem.org/wiki/images/2/21/Team_SDU_Denmark_Growth_rate_assay_2_PS.zip Raw data] ]]<br><br>
[[Image:Team SDU-Denmark OD WT+PS.JPG|thumb|center|400px|Growth curve showing measured OD at 550nm. Samples were taken every hour over a 12 hour perion + one sample after 24 hours. In graph ''E. coli'' strain MG1655-pSB3T5-k343007 and MG1655-pSB1C3-K343007 is compaired with Wild type ''E. coli'' strain MG1655 showing no significant difference between the three graphs. No Lag phase is seen. All data can be seen under [http://2010.igem.org/wiki/images/2/21/Team_SDU_Denmark_Growth_rate_assay_2_PS.zip Raw data] ]]<br><br>
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343007 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth are not significantly influenced by the plasmid. The added reproduction load due to the plasmids, might also prolong the lag phase of the bacteria. Whether this is the case can not be concluded based on this experiment as no lag phase was observed in this experiment.<br><br>
From our data we see no significant difference between the plasmid carrying bacteria and the wild type. This can be said to be quite conteradictory to our results obtained from the stability assay. The transitory stability of pSB1C3-K343007 suggests that it is highly unfavorable for the bacteria, wherefore it might be expected that the growth of the bacteria containg this plasmid would be affected. Thus, however much a disadvantage the plasmid pose to the bacteria, their growth are not significantly influenced by the plasmid. The added reproduction load due to the plasmids, might also prolong the lag phase of the bacteria. Whether this is the case can not be concluded based on this experiment as no lag phase was observed in this experiment.<br><br>
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= References =
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[1] Derek L. Englert, Arul Jayaraman, Michael D. Manson,[http://www.springerlink.com/content/n386247071624387/fulltext.pdf Methods in Molecular Biology], 2009, Volume 571, 1-23.<br>
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[2] Jung KH, Spudich EN, Trivedi VD, Spudich JL, [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC100132/pdf/jb2101006365.pdf An Archaeal Photosignal-Transducing Module Mediates Phototaxis in Escherichia coli] J Bacteriol. 2001 Nov;183(21)<br>
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Revision as of 23:56, 27 October 2010