Team:SDU-Denmark/K343007

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== K343007 ==
== K343007 ==
The part K343007 (from now on shortly called PS) is a generator for the SopII-HtrII photosensor from ''Natronomonas pharaonis'' coupled to ''E. Coli's'' chemotaxis pathway via the ''Salmonella enterica'' protein Tar. This part's effect on the system is to make ''E. Coli'' phototactic, so that it becomes aware of different light conditions. So for characterization of this part we made examination of motility and motility patterns our first priority and plasmid stability and growth of the cells our second. <br>
The part K343007 (from now on shortly called PS) is a generator for the SopII-HtrII photosensor from ''Natronomonas pharaonis'' coupled to ''E. Coli's'' chemotaxis pathway via the ''Salmonella enterica'' protein Tar. This part's effect on the system is to make ''E. Coli'' phototactic, so that it becomes aware of different light conditions. So for characterization of this part we made examination of motility and motility patterns our first priority and plasmid stability and growth of the cells our second. <br>
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There is a wide range of motility assays for chemotaxis in bacteria, this meant that we had a broad spectrum of experiments to choose from, which just had to be tweaked for making them suited for the analysis of phototaxis. The two experiments we chose for analysing the effect of this part (PS), were growth of the bacterial cultures in semi-solid agar and computer analysis of swimming motility through video microscopy:<br>
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There is a wide range of motility assays for chemotaxis in bacteria, this meant that we had a broad spectrum of experiments to choose from, which just had to be tweaked for making them suited for the analysis of phototaxis. The two experiments we chose for analysing the effect of this part (PS), were growth of the bacterial cultures in semi-solid agar and computer analysis of swimming motility through video microscopy.<br>
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=== Growth of bacterial culture on semi-solid agar plates===
=== Growth of bacterial culture on semi-solid agar plates===

Revision as of 23:28, 27 October 2010