Team:SDU-Denmark/K343006

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(Difference between revisions)
(Growth assay)
(UV-Vis determination of beta-carotene and retinal production)
 
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MG1655-pSB1A2-K274210/pSB1C3-K343005 (double transformants)<br>
MG1655-pSB1A2-K274210/pSB1C3-K343005 (double transformants)<br>
Both K343005 and K274210 were constitutively expressed.<br><br>
Both K343005 and K274210 were constitutively expressed.<br><br>
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Since the first experiment showed no or small amounts of product in cells grown to exponential phase the measurements were performed on cells incubated for 20 hours at 37 degrees Celcius and 180rpm. The graphs obtained from the experiment are presented below:<br> <br>
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Since the first experiment showed no or small amounts of product in cells grown to exponential phase the measurements were performed on cells incubated for 20 hours at 37 degrees Celcius. The graphs obtained from the experiment are presented below:<br> <br>
[[Image: Team-SDU-denmark-Samples form top10 mg1655 and control.png |thumb|400px|center|'''Figure 1:''' UV-VIS spectre of six ''E. coli'' strains: Wild type TOP10, Wild type MG1655, TOP10-pSB1A2-K274210, MG1655-pSB1A2-K274210, TOP10-pSB1A2-K274210/pSB1C3-K343005 (double transformants), MG1655-pSB1A2-K274210/pSB1C3-K343005 (double transformants)]]
[[Image: Team-SDU-denmark-Samples form top10 mg1655 and control.png |thumb|400px|center|'''Figure 1:''' UV-VIS spectre of six ''E. coli'' strains: Wild type TOP10, Wild type MG1655, TOP10-pSB1A2-K274210, MG1655-pSB1A2-K274210, TOP10-pSB1A2-K274210/pSB1C3-K343005 (double transformants), MG1655-pSB1A2-K274210/pSB1C3-K343005 (double transformants)]]
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Latest revision as of 01:46, 28 October 2010