Team:SDU-Denmark/K343006

From 2010.igem.org

(Difference between revisions)
(K343005)
(HPLC determination of beta-carotene and retinal production)
Line 44: Line 44:
MG1655 containing PSB1A2 with K274210 plus PSB1C3 with K343005 both under a constitutively active promotor<br>  
MG1655 containing PSB1A2 with K274210 plus PSB1C3 with K343005 both under a constitutively active promotor<br>  
The measurements were preformed on cells after 20 hours of growth. The resulting graphs is presented beneath the text.<br>
The measurements were preformed on cells after 20 hours of growth. The resulting graphs is presented beneath the text.<br>
-
[[Image:Team SDU-Denmark-HPLC samples2.png|thumb|left|300px|HPLC analysis of samples from the 19th oct. 2010 Beta-carotene Peaks are clearly present, but no retinal peaks are observed. Measurements were made after 20 hours of growth]]
+
[[Image:Team SDU-Denmark-HPLC samples2.png|thumb|center|300px|HPLC analysis of samples from the 19th oct. 2010 Beta-carotene Peaks are clearly present, but no retinal peaks are observed. Measurements were made after 20 hours of growth]]
[[Image:Team SDU-Denmark-HPLC samples1.png|thumb|center|300px|HPLC analysis of samples from the 22nd oct. 2010 Beta-carotene Peaks are clearly pressent, but no retinal peaks are observed. Measurements were made after 20 hours of growth]]<br>
[[Image:Team SDU-Denmark-HPLC samples1.png|thumb|center|300px|HPLC analysis of samples from the 22nd oct. 2010 Beta-carotene Peaks are clearly pressent, but no retinal peaks are observed. Measurements were made after 20 hours of growth]]<br>
When looking at the retinal and beta-carotene standards analysed on the HPLC, peaks for both chemical are clearly present. The retinal retention time is 3,480-3,490 minutes and the beta-carotene retention time is 23,300-23,600 minutes.<br>     
When looking at the retinal and beta-carotene standards analysed on the HPLC, peaks for both chemical are clearly present. The retinal retention time is 3,480-3,490 minutes and the beta-carotene retention time is 23,300-23,600 minutes.<br>     

Revision as of 00:42, 28 October 2010