Team:SDU-Denmark/K343004

From 2010.igem.org

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(Phase contrast of ON cultures)
(Phase contrast of ON cultures)
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=== Phase contrast of ON cultures===
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The purpose of the phase contrast microscopy is to see if the transformants are visibly different from the wild type. Since the FlhDC operon is not only an important part of the flagella synthesis but is also coupled to cell metabolism [[http://2010.igem.org/Team:SDU-Denmark/project-p#References 3]], it might be possible to see difference in morphology caused by an alterations in the cell cycle. <br>
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Phase contrast pictures were taken of wild type MG1655, MG1655/pSB1C3-K343004 and MG1655/pSB3K3-K343004.<br><br>
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[[Image:Team-SDU-Denmark-Flagella-PCM-1.jpg|630px|center|thumb]]<br><br>
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The pictures clearly show a difference in morphology of the wild type (picture 1) and the two transformants (picture 2-pSB1C3 and 3-pSB3K3). The wild type cells are small cells, while the transformants are obviously larger, which indicates a lower metabolism of the transformants. Cells with impaired metabolism divide slower and are larger than cells with normal metabolism (wild type), since it takes the impaired cells longer to reach the mitotic phase of the cell cycle.<br>
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= References =
= References =
[1] Clive S. Barker, Birgit M. Prüß, and Philip Matsumura [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC524886/pdf/0575-04.pdf Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon]
[1] Clive S. Barker, Birgit M. Prüß, and Philip Matsumura [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC524886/pdf/0575-04.pdf Increased Motility of Escherichia coli by Insertion Sequence Element Integration into the Regulatory Region of the flhD Operon]

Revision as of 00:53, 28 October 2010