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Team:RMIT Australia/Notebook/Media
From 2010.igem.org
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=='''Media Recipes '''== | =='''Media Recipes '''== | ||
| - | '''SOC Medium''' | + | '''SOC Medium'''<br> |
4g Tryptone<br> | 4g Tryptone<br> | ||
1g Yeast extract<br> | 1g Yeast extract<br> | ||
| Line 438: | Line 438: | ||
Deionised water up to 190ml<br> | Deionised water up to 190ml<br> | ||
Then autoclave | Then autoclave | ||
| - | + | <br> | |
| - | '''LB Media''' | + | '''LB Media'''<br> |
| - | 5g Tryptone | + | 5g Tryptone<br> |
| - | 2.5 g yeast extract | + | 2.5 g yeast extract<br> |
| - | 5g NaCl | + | 5g NaCl<br> |
| - | 500ml deionised water | + | 500ml deionised water <br> |
Then autoclave | Then autoclave | ||
Revision as of 04:43, 23 September 2010
Media Recipes
SOC Medium
4g Tryptone
1g Yeast extract
500mg NaCl
2ml 250mM KCl
Deionised water up to 190ml
Then autoclave
LB Media
5g Tryptone
2.5 g yeast extract
5g NaCl
500ml deionised water
Then autoclave
