Team:RMIT Australia/Notebook

From 2010.igem.org

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(Notebook)
(Notebook)
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<div id=" .2F15_September_2010">'''19 September'''  
<div id=" .2F15_September_2010">'''19 September'''  
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- sent off project abstract to HQ
- sent off project abstract to HQ

Revision as of 11:19, 19 September 2010


Notebook

May
MTWTFSS
          1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31


28 June

Did OH&S training and PC2 training

29 June

Began research on what we were going to use to promote taq

30 June

Looked at T7 with a Lac operon as a promotore of taq

13 July

Designed Primers for Taq into backbone

Researched about using an Asp/Pro between the peptide and the Taq

Began looking at ligation independent cloning for insertion of peptide

14 July

Looked at prices of Hotels in Boston

19 July

began looking at the structure of taq, its thermostability began looking at different mutations that can be done to taq to increase its thermostability without changing its structure

21 July

sent in sequence of wild type taq to iTasser

23 July

Checked thermostability of the mutations that will be done to Taq using Expasy

Sent in sequence of taq with mutations at 485, 515 and 540 to iTasser

3 August

Had iGEM team meeting

-Discussed ANZAAS Proposal

-Spoke about possibilities of using Geneart

-Discussed possible T-shirt designs

5 August

Meet up with Damian, discussed different strategies to use in modelling, began using YASARA to do our Modelling

10 August

Had iGEM team meeting

-Took Team Photo

11 August

PCR of Taq

Taq with a DNA concentration of 40ng/µL was diluted to 1µM by addition of 24µL of MilliQ water to 1µL of the Taq plasmid. Primers were diluted to 100µM by adding 354µL to the forward primer with a concentration of 35.4 nmol and 405µL to the reverser primer with a concentration of 40.5nmol. The primers were further diluted to 0.2µM by adding 10µL of the primer to 490µL of milliQ water. The PCR reaction was then made by adding 25µL of Master Mix, 2.5µL of each primer, 5µL of Rnase free water and 15µL of 0.2µM Taq Plasmid.

The PCR

98º 60 Sec 1 cycle

98º 15 Sec then 72º 90 Sec 25 cycle

72º 5 min 1 cycle

16 August

Collaborated with the Sheffield team by participating in an over the phone survery about Human Practices

17 August

had team meeting

- discussed how we can get more sponsors

- talked about placing our order to gene art

- began filling out the form to send Pennstates survey to the ethics committee

- discussed results for modelling

- decided to fix optimise our previous PCR

18 August

called few companies seeking sponsorship enquired for booking flights and accomadation

19 August

Working on comparing TAQ wild structure to the TAQ Mutated structure to see that there are no major differences, which will mean that TAQ is inactive but will retain it's thermostability at high temperatures.

Wrote an email to ABC TV, wanting to know if they will do piece on our project for there The New Inventors Program.

Waiting for results from I-Tasser on the mutated TAQ sequence. Went into the lab and made SOC media and LB- agar plates

20 August

Did two PCR reaction and a transformation The first PCR we ran was a touch down PCR. Temperatures ranged from 69 to 64. The second PCR was a two step PCR.

30 August

Ordered the TAQ polymerase from Mr. Gene. Should have it in 15 days. Meeting today at 10am to discuss project budget and costings. After the meeting we all are sitting together for a writing session.

31 August

Spent the morning organising our flights In the afternoon we all sat together for a writing session

1 September

We all sat together for another writing session.

7 September

Had a team meeting

14 September

Had a team meeting and made a lab time table, we also set out tasks which need to be done by the next meeting - Update the wiki

- Finalise the form for the ethics committee for pennstate

- Call up possible sponsors

- Collect Tshirts

- Work on abstract and team Roster

- research heat liable bonds

- order peptides

- chat with emilio about possible bio assays

- work on making the promotor part

15 September

went into the lab....

17 September

- finalized project abstract

19 September


- sent off project abstract to HQ