From 2010.igem.org
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| + | <div id=" .2F18_August_2010">'''18 August''' |
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| + | called few companies seeking sponsorship |
| + | enquired for booking flights and accomadation |
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Revision as of 04:14, 18 August 2010
Notebook
3 August
Had iGEM team meeting
-Discussed ANZAAS Proposal
-Spoke about possibilities of using Geneart
-Discussed possible T-shirt designs
10 August
Had iGEM team meeting
-Took Team Photo
11 August
PCR of Taq
Taq with a DNA concentration of 40ng/µL was diluted to 1µM by addition of 24µL of MilliQ water to 1µL of the Taq plasmid. Primers were diluted to 100µM by adding 354µL to the forward primer with a concentration of 35.4 nmol and 405µL to the reverser primer with a concentration of 40.5nmol. The primers were further diluted to 0.2µM by adding 10µL of the primer to 490µL of milliQ water. The PCR reaction was then made by adding 25µL of Master Mix, 2.5µL of each primer, 5µL of Rnase free water and 15µL of 0.2µM Taq Plasmid.
The PCR
98º 60 Sec 1 cycle
98º 15 Sec then 72º 90 Sec 25 cycle
72º 5 min 1 cycle
18 August
called few companies seeking sponsorship
enquired for booking flights and accomadation
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