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Team:RMIT Australia/Modeling
From 2010.igem.org
(→Taq DNA Polymerase) |
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= Modelling = | = Modelling = | ||
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| + | == Parameters used to compare the Models == | ||
| + | [[Image:Table.jpg]] | ||
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== Taq DNA Polymerase == | == Taq DNA Polymerase == | ||
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Taq Polymerase with no mutations | Taq Polymerase with no mutations | ||
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The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins. | The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins. | ||
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Our Taq Polymerase with mutations | Our Taq Polymerase with mutations | ||
[[Image:Taq with labels.jpg]] | [[Image:Taq with labels.jpg]] | ||
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| + | The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq at the N and C terminus. | ||
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| + | [[Image:Taq N.jpg|400px]] [[Image:Taq C.jpg|400px]] | ||
== Fold-it== | == Fold-it== | ||
Revision as of 08:21, 11 October 2010
Modelling
Parameters used to compare the Models
Taq DNA Polymerase
Taq Polymerase with no mutations
Aliphatic Index: 98.33
Ext Coefficient: 112760
Theoretical PI: 6.04
Instability Index: 32.93
We redesigned TAQ so it is suitable to undergo ligation independent cloning, to do this we changed the N and C terminal.
This is the redesigned Taq Polymerase
Aliphatic Index: 96.46
Ext Coefficient: 107260
Theoretical PI: 6.09
Instability Index: 33.39
The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.
Our Taq Polymerase with mutations
The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq at the N and C terminus.
Fold-it
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