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Team:RMIT Australia/Modeling
From 2010.igem.org
(→Taq DNA Polymerase) |
(→Taq DNA Polymerase) |
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| - | We redesigned TAQ so it | + | We redesigned TAQ so it is suitable to undergo ligation independent cloning, to do this we changed the N and C terminal. |
This is the redesigned Taq Polymerase | This is the redesigned Taq Polymerase | ||
| Line 442: | Line 442: | ||
'''Instability Index:''' 33.39 | '''Instability Index:''' 33.39 | ||
| + | |||
| + | |||
| + | The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins. | ||
| + | |||
| + | The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq. | ||
| + | |||
| + | (Green is the redesigned Taq and Pink is the wild type Taq) | ||
| + | |||
| + | [[Image:comaprison.jpg|400px]] | ||
| + | |||
| + | ---- | ||
== Fold-it== | == Fold-it== | ||
Revision as of 04:53, 31 August 2010
Modelling
Taq DNA Polymerase
Taq Polymerase with no mutations
Aliphatic Index: 98.33
Ext Coefficient: 112760
Theoretical PI: 6.04
Instability Index: 32.93
We redesigned TAQ so it is suitable to undergo ligation independent cloning, to do this we changed the N and C terminal.
This is the redesigned Taq Polymerase
Aliphatic Index: 96.46
Ext Coefficient: 107260
Theoretical PI: 6.09
Instability Index: 33.39
The wild type Taq Polymerase was changed at the N and C terminal, with the Root Mean Square Deviation of 6.8834. This number indicates the measure of the average distance between the backbones of the two superimposed proteins.
The picture below shows the differences between the wild type Taq Polymerase and the redesigned Taq.
(Green is the redesigned Taq and Pink is the wild type Taq)
Fold-it
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