Team:Queens-Canada/parts

From 2010.igem.org

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<html><div class="section"><h2>Regulatory</h2></html>
<html><div class="section"><h2>Regulatory</h2></html>
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All but one of the regulatory elements we isolated are promoters, and are described in brief detail below, with more elaborate information on their parts registry pages. The remaining BioBrick is the 3' UTR from ''unc-54'', which is roughly equivalent to a bacterial terminator and performs a number of important regulatory functions in complement to the promoter. All complete ''C. elegans'' constructs must include some form of functional 3' UTR.
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All but one of the regulatory elements we isolated are promoters, and are described in brief detail below, with more elaborate information on their parts registry pages. The remaining BioBrick is the 3' UTR from ''unc-54'', which is roughly equivalent to a bacterial terminator and performs a number of important regulatory functions in complement to the promoter. All complete ''C. elegans'' constructs must include some form of functional 3' UTR. Promoters were selected based on their utility, strength of expression, and ease of avoiding potentially harmful cutsites.
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<h3>Constitutive Promoters</h3>
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<h3>Constitutive</h3>
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* '''pGpd-2''':
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* '''pGpd-2''': ''gpd-2'' is part of the glycolysis pathway. The gpd-2 promoter thus expresses at a very high level—AceView <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=gpd-2&submit=Go">says 43.5 times the average</a></html> for a ''C. elegans'' constitutive gene.
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* '''pHsp-3''':
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* '''pHsp-3''': HSP-3 is a protein involved in the heat shock response pathway, but it is expressed constitutively. Aceview <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=hsp-3&submit=Go">says this is 22.9 times the average</a></html>.
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* '''p''':
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* '''pRab-7''': ''rab-7'' expresses a GTPase involved in endosome trafficking, and expresses at <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/av.cgi?exdb=AceView&db=worm&term=rab-7&submit=Go">4.3 times the average</a></html>.
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* '''p''':
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<h3>Inducible Promoters</h3>
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<h3>Inducible</h3>
* '''pSip-1''':
* '''pSip-1''':
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* '''p''':
 
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* '''p''':
 
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* '''p''':
 
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<h3>Tissue-Specific Promoters</h3>
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<h3>Tissue-Specific</h3>
* '''pMec-7''':
* '''pMec-7''':
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* '''p''':
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* '''pOdr-1''':
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* '''p''':
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* '''pStr-1''':
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* '''p''':
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* '''pOsm-10''':
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* '''p''':
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* '''pFlp-1''':
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* '''p''':
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* '''pSra-10''':
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* '''p''':
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* '''pStr-220''':
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* '''p''':
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<html></div><div class="section"><h2>Reporters</h2></html>
<html></div><div class="section"><h2>Reporters</h2></html>
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* '''eYFP''':
* '''eYFP''':
* '''mCherry''':
* '''mCherry''':
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* ''Constructs'':
<html></div><div class="section"><h2>Optogenetics Proteins</h2></html>
<html></div><div class="section"><h2>Optogenetics Proteins</h2></html>

Revision as of 05:43, 27 October 2010

WormWorks Parts List

Regulatory

All but one of the regulatory elements we isolated are promoters, and are described in brief detail below, with more elaborate information on their parts registry pages. The remaining BioBrick is the 3' UTR from unc-54, which is roughly equivalent to a bacterial terminator and performs a number of important regulatory functions in complement to the promoter. All complete C. elegans constructs must include some form of functional 3' UTR. Promoters were selected based on their utility, strength of expression, and ease of avoiding potentially harmful cutsites.

Constitutive

  • pGpd-2: gpd-2 is part of the glycolysis pathway. The gpd-2 promoter thus expresses at a very high level—AceView says 43.5 times the average for a C. elegans constitutive gene.
  • pHsp-3: HSP-3 is a protein involved in the heat shock response pathway, but it is expressed constitutively. Aceview says this is 22.9 times the average.
  • pRab-7: rab-7 expresses a GTPase involved in endosome trafficking, and expresses at 4.3 times the average.

Inducible

  • pSip-1:

Tissue-Specific

  • pMec-7:
  • pOdr-1:
  • pStr-1:
  • pOsm-10:
  • pFlp-1:
  • pSra-10:
  • pStr-220:

Reporters

  • eGFP:
  • eCFP:
  • eYFP:
  • mCherry:
  • Constructs:

Optogenetics Proteins

  • ChR2:
  • NpHR:
  • Fusions: ChR2::eYFP and NpHR::eCFP

Getting the Parts

You can get our special worm parts through the standard iGEM distribution channel: the Parts Registry. Click on a part number below to be taken to the relevant description page.

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