Team:Queens-Canada/parts

From 2010.igem.org

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{{:Team:Queens-Canada/head}}
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=Part Structure=
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<h1>WormWorks Parts List</h1>
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<html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry].
<html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry].
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<div id="groupparts" style="display: none;">
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<div id="groupparts" style="display: block;">
<groupparts>iGEM010 Queens-Canada</groupparts>
<groupparts>iGEM010 Queens-Canada</groupparts>
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'''Note:''' The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.
 
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=General=
 
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==Promoters==
 
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{|
 
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!Name
 
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!Description
 
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!Strength
 
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|-
 
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|''sip-1''
 
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|Heat shock
 
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|
 
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|''rab-7''
 
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|Constitutive
 
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|high
 
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|''mec-7''
 
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|Mechanosensory neurons
 
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|
 
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|''hsp-3''
 
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|Heat shock
 
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|
 
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|''gpd-2''
 
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|Constitutive
 
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|very high
 
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|''unc-47''
 
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|GABA-using (gabergic) neurons
 
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|
 
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|}
 
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==Coding Sequences==
 
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{|
 
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!Name
 
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!Description
 
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|GFP
 
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|Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria.
 
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|eGFP
 
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|Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691.
 
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|mCherry
 
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|Red Fluorescent Protein. From Chin-Sang plasmid.
 
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|YFP
 
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|Yellow Fluorescent Protein. From Chin-Sang plasmid.
 
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|eCFP
 
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|Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.
 
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|}
 
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==Backbones==
 
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* Since it is possible to microinject plasmids into ''C. elegans'', we're working on a combination backbone that will produce GFP in both ''E. coli'' and ''C. elegans'', as well as providing antibiotic selection markers for ''E. coli'', and the cutsites from Standard 10.
 
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* Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.
 
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=Optogenetics (R.''C. elegans'')=
 
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==Promoters==
 
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{|
 
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!Name
 
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!Description
 
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!Strength
 
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|}
 
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==Coding Squences==
 
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{|
 
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!Name
 
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!Description
 
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|ChR2
 
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|Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells.
 
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|NpHR
 
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|Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing.
 
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|}
 
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Revision as of 18:23, 26 October 2010

WormWorks Parts List

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