"
Team:Queens-Canada/parts
From 2010.igem.org
(Difference between revisions)
m (Team:Queens-Canada/Parts moved to Team:Queens-Canada/parts) |
|||
| Line 3: | Line 3: | ||
=Part Structure= | =Part Structure= | ||
| + | <html><script type="text/javascript"> | ||
| + | function toggle_visibility(id) { | ||
| + | el = document.getElementById(id); | ||
| + | if(el.style.display == "none") { | ||
| + | el.style.display = "block"; | ||
| + | } else { | ||
| + | el.style.display = "none"; | ||
| + | } | ||
| + | }</script> | ||
| + | </html> | ||
<div class="box"> | <div class="box"> | ||
<html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry]. | <html><a href="#" onclick="toggle_visibility('groupparts');">Show/hide parts</a></html> as entered in the [http://partsregistry.org Registry]. | ||
Revision as of 18:22, 26 October 2010
Part Structure
Show/hide parts as entered in the Registry.
Note: The following list is tentative, and better reflects our goals rather than our current accomplishments. We are still in the process of isolating and characterising these components.
General
Promoters
| Name | Description | Strength |
|---|---|---|
| sip-1 | Heat shock | |
| rab-7 | Constitutive | high |
| mec-7 | Mechanosensory neurons | |
| hsp-3 | Heat shock | |
| gpd-2 | Constitutive | very high |
| unc-47 | GABA-using (gabergic) neurons |
Coding Sequences
| Name | Description |
|---|---|
| GFP | Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria. |
| eGFP | Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691. |
| mCherry | Red Fluorescent Protein. From Chin-Sang plasmid. |
| YFP | Yellow Fluorescent Protein. From Chin-Sang plasmid. |
| eCFP | Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid. |
Backbones
- Since it is possible to microinject plasmids into C. elegans, we're working on a combination backbone that will produce GFP in both E. coli and C. elegans, as well as providing antibiotic selection markers for E. coli, and the cutsites from Standard 10.
- Another project we're pursuing is the possible usage of double-stranded RNA as a liaison for DNA import. If this works, we'll have hybrid strand backbones for that method of transformation as well.
Optogenetics (R.C. elegans)
Promoters
| Name | Description | Strength |
|---|
Coding Squences
| Name | Description |
|---|---|
| ChR2 | Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells. |
| NpHR | Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing. |
