Team:Queens-Canada/parts

From 2010.igem.org

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(Promoters)
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!Strength
!Strength
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|sip-1
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|''sip-1''
|Heat shock
|Heat shock
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|rab-7
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|''rab-7''
|Constitutive
|Constitutive
|high
|high
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|mec-7
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|''mec-7''
|Mechanosensory neurons
|Mechanosensory neurons
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|hsp-3
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|''hsp-3''
|Heat shock
|Heat shock
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|gpd-2
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|''gpd-2''
|Constitutive
|Constitutive
|very high
|very high
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|unc-47
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|''unc-47''
|GABA-using (gabergic) neurons
|GABA-using (gabergic) neurons
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Revision as of 21:21, 22 June 2010

Part Structure

Show/hide parts as entered in the Registry.

General

Promoters

Name Description Strength
sip-1 Heat shock
rab-7 Constitutive high
mec-7 Mechanosensory neurons
hsp-3 Heat shock
gpd-2 Constitutive very high
unc-47 GABA-using (gabergic) neurons

Coding Sequences

Name Description
GFP Enhanced Green Fluorescent Protein. From biobrick BBa_I714891. Proof of concept to see if genes can be imported from bacteria.
eGFP Enhanced Green Fluorescent Protein. From Chin-Sang plasmid pIC691.
mCherry Red Fluorescent Protein. From Chin-Sang plasmid.
YFP Yellow Fluorescent Protein. From Chin-Sang plasmid.
eCFP Enhanced Cyan Fluorescent Protein. From Chin-Sang plasmid.

Optogenetics (R.C. elegans)

Promoters

Name Description Strength

Coding Squences

Name Description
ChR2 Channelrhodopsin-2. Permits entry of positive ions in response to 460 nm light. Can be used to trigger nerve cells.
NpHR Halorhodopsin. Permits entry of chloride ions in response to 580 nm light. Can be used to block nerve cells from firing.