http://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&feed=atom&action=historyTeam:Queens-Canada/idea - Revision history2024-03-29T01:20:34ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=171896&oldid=prevGlh at 02:39, 27 October 20102010-10-27T02:39:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Objective</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Objective</h1></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our <del class="diffchange diffchange-inline">main </del>goal <del class="diffchange diffchange-inline">for </del>this summer was to build a toolkit for the nematode ''Caenorhabditis elegans'' <del class="diffchange diffchange-inline">for </del>use as a synthetic biology chassis. Our focus was on finding and selecting viable promoters, protein domains, and other components for use by future teams.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our goal this summer was to build a toolkit for the nematode ''Caenorhabditis elegans'' <ins class="diffchange diffchange-inline">to facilitate its future </ins>use as a synthetic biology chassis. Our focus was on finding and selecting viable promoters, protein domains, and other <ins class="diffchange diffchange-inline">key </ins>components for use by future teams.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The motivation for our project is simple enough: to date, there has been little interest in using complex eukaryotic and multicellular organisms in iGEM, and we feel that the advantages of multicellular organisms outweigh the challenges involved in working with them.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The motivation for our project is simple enough: to date, there has been little interest in using complex eukaryotic and multicellular organisms in iGEM, and we feel that the advantages of multicellular organisms outweigh the challenges involved in working with them<ins class="diffchange diffchange-inline">. With the benefit of the documentation and components we hope that most of these issues</ins>.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">We really want </del>to <del class="diffchange diffchange-inline">encourage other </del>teams to <del class="diffchange diffchange-inline">embrace </del>the <del class="diffchange diffchange-inline">potential </del>benefits of working in a complicated organism like ''C. elegans''<del class="diffchange diffchange-inline">, which </del>has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, <del class="diffchange diffchange-inline">it </del>represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The jump from working with ''E. coli'' </ins>to <ins class="diffchange diffchange-inline">''C. elegans'' not an insurmountable one; the worm has a short generation time, is grown at room temperature on agar plates, can be frozen, and it can be transformed using standard BioBrick plasmids assembled in ''E. coli''. </ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Through WormGuide, future </ins>teams <ins class="diffchange diffchange-inline">will be able </ins>to <ins class="diffchange diffchange-inline">easily and quickly learn all about </ins>the <ins class="diffchange diffchange-inline">worm and the possibilities that it holds. The parts that we have submitted will then provide them with a solid base from which they can build their projects.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The </ins>benefits of working in a complicated organism like ''C. elegans'' <ins class="diffchange diffchange-inline">are significant. It </ins>has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, <ins class="diffchange diffchange-inline">the worm </ins>represents one of the best <ins class="diffchange diffchange-inline">and most accessible </ins>opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/">Channelrhodopsin-2, a directly light-gated cation-selective membrane channel</a></html>. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/">Channelrhodopsin-2, a directly light-gated cation-selective membrane channel</a></html>. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td></tr>
</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=149043&oldid=prevGlh at 20:49, 25 October 20102010-10-25T20:49:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By combining these proteins in a single transgenic organism, behind specifically-chosen promoters which target only certain neurons, we hope to make a "remote control" worm, which can be instructed to move either forward or backward by exposing it to the appropriate wavelengths of light.<html></div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By combining these proteins in a single transgenic organism, behind specifically-chosen promoters which target only certain neurons, we hope to make a "remote control" worm, which can be instructed to move either forward or backward by exposing it to the appropriate wavelengths of light.<html></div></html></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> #footnotes p {</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/">Channelrhodopsin-2, a directly light-gated cation-selective membrane channel</a></html>. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 <html><a target="_new" href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/">Channelrhodopsin-2, a directly light-gated cation-selective membrane channel</a></html>. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|2|Han X, Boyden ES, 2007 <html><a target="_new" href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299">Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution</a></html>. PLoS ONE 2(3): e299. doi:10.1371/journal.pone.0000299}} </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|2|Han X, Boyden ES, 2007 <html><a target="_new" href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299">Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution</a></html>. PLoS ONE 2(3): e299. doi:10.1371/journal.pone.0000299}} </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td></tr>
</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=149027&oldid=prevGlh at 20:48, 25 October 20102010-10-25T20:48:29Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Footnotes and Citations</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Footnotes and Citations</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 <del class="diffchange diffchange-inline">[</del>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel<del class="diffchange diffchange-inline">]</del>. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 <ins class="diffchange diffchange-inline"><html><a target="_new" href="</ins>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/<ins class="diffchange diffchange-inline">"></ins>Channelrhodopsin-2, a directly light-gated cation-selective membrane channel<ins class="diffchange diffchange-inline"></a></html></ins>. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|2|Han X, Boyden ES, 2007 <del class="diffchange diffchange-inline">[</del>http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299 Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution<del class="diffchange diffchange-inline">]</del>. PLoS ONE 2(3): e299. doi:10.1371/journal.pone.0000299}} </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|2|Han X, Boyden ES, 2007 <ins class="diffchange diffchange-inline"><html><a target="_new" href="</ins>http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299<ins class="diffchange diffchange-inline">"></ins>Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution<ins class="diffchange diffchange-inline"></a></html></ins>. PLoS ONE 2(3): e299. doi:10.1371/journal.pone.0000299}} </div></td></tr>
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</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=136332&oldid=prevGlh at 19:10, 24 October 20102010-10-24T19:10:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We really want to encourage other teams to embrace the potential benefits of working in a complicated organism like ''C. elegans'', which has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, it represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We really want to encourage other teams to embrace the potential benefits of working in a complicated organism like ''C. elegans'', which has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, it represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><html><div class="section"><h2>R.<del class="diffchange diffchange-inline">''</del>C. elegans<del class="diffchange diffchange-inline">''</del></h2></html></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><html><div class="section"><h2>R.<ins class="diffchange diffchange-inline"><i></ins>C. elegans<ins class="diffchange diffchange-inline"></i></ins></h2></html></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One diagnostic tool which has received a lot of attention recently are the '''channelrhodopsins''', which were isolated from the green algae ''Chlamydomonas reinhardtii'', and serve as light-activated nonspecific cation channels which can cause nerve cells to depolarise in response to certain wavelengths of light. These have been used extensively in research biology to interrogate the roles of the neurons in ''C. elegans'' and other species, allowing researchers to, for example, cause nematodes to contract all of their muscles at once and seize up in response to specific wavelengths of light.{{:Team:Queens-Canada/footnote-anchor|1}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One diagnostic tool which has received a lot of attention recently are the '''channelrhodopsins''', which were isolated from the green algae ''Chlamydomonas reinhardtii'', and serve as light-activated nonspecific cation channels which can cause nerve cells to depolarise in response to certain wavelengths of light. These have been used extensively in research biology to interrogate the roles of the neurons in ''C. elegans'' and other species, allowing researchers to, for example, cause nematodes to contract all of their muscles at once and seize up in response to specific wavelengths of light.{{:Team:Queens-Canada/footnote-anchor|1}}</div></td></tr>
</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=135644&oldid=prevGlh at 18:09, 24 October 20102010-10-24T18:09:25Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our main goal for this summer <del class="diffchange diffchange-inline">is </del>to build a toolkit for the nematode ''Caenorhabditis elegans'' for use as a synthetic biology chassis. Our focus <del class="diffchange diffchange-inline">is </del>on finding and selecting viable promoters, protein domains, and other components for use by future teams.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our main goal for this summer <ins class="diffchange diffchange-inline">was </ins>to build a toolkit for the nematode ''Caenorhabditis elegans'' for use as a synthetic biology chassis. Our focus <ins class="diffchange diffchange-inline">was </ins>on finding and selecting viable promoters, protein domains, and other components for use by future teams.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The motivation for our project is simple enough: to date, there has been little interest in using complex eukaryotic and multicellular organisms in iGEM, and we feel that the advantages of multicellular organisms outweigh the challenges involved in working with them.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The motivation for our project is simple enough: to date, there has been little interest in using complex eukaryotic and multicellular organisms in iGEM, and we feel that the advantages of multicellular organisms outweigh the challenges involved in working with them.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The main focus of our project is encouraging </del>other teams to embrace the potential benefits of working in a complicated organism like ''C. elegans'', which has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, it represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We really want to encourage </ins>other teams to embrace the potential benefits of working in a complicated organism like ''C. elegans'', which has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, it represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h2>R.''C. elegans''</h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><html><div class="section"></ins><h2>R.''C. elegans''</h2<ins class="diffchange diffchange-inline">></html</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One diagnostic tool which has received a lot of attention recently are the '''channelrhodopsins''', which were isolated from the green algae ''Chlamydomonas reinhardtii'', and serve as light-activated nonspecific cation channels which can cause nerve cells to depolarise in response to certain wavelengths of light. These have been used extensively in research biology to interrogate the roles of the neurons in ''C. elegans'' and other species, allowing researchers to, for example, cause nematodes to contract all of their muscles at once and seize up in response to specific wavelengths of light.{{:Team:Queens-Canada/footnote-anchor|1}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One diagnostic tool which has received a lot of attention recently are the '''channelrhodopsins''', which were isolated from the green algae ''Chlamydomonas reinhardtii'', and serve as light-activated nonspecific cation channels which can cause nerve cells to depolarise in response to certain wavelengths of light. These have been used extensively in research biology to interrogate the roles of the neurons in ''C. elegans'' and other species, allowing researchers to, for example, cause nematodes to contract all of their muscles at once and seize up in response to specific wavelengths of light.{{:Team:Queens-Canada/footnote-anchor|1}}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Acting as counterpart to the channelrhodopsins is a channel called '''halorhodopsin''', isolated from halophilic archaeans, which causes the cell to uptake chloride anions, and thus can be used to hyperpolarise a neuron and prevent it from firing. Importantly, the halorhodopsin excitation frequency is well-separated from the excitation frequency of channelrhodopsin-2, and so they can be used in the same organism, or even the same cell, without significant cross-talk.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Acting as counterpart to the channelrhodopsins is a channel called '''halorhodopsin''', isolated from halophilic archaeans, which causes the cell to uptake chloride anions, and thus can be used to hyperpolarise a neuron and prevent it from firing. Importantly, the halorhodopsin excitation frequency is well-separated from the excitation frequency of channelrhodopsin-2, and so they can be used in the same organism, or even the same cell, without significant cross-talk.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>By combining these proteins in a single transgenic organism, behind specifically-chosen promoters which target only certain neurons, we hope to make a "remote control" worm, which can be instructed to move either forward or backward by exposing it to the appropriate wavelengths of light.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>By combining these proteins in a single transgenic organism, behind specifically-chosen promoters which target only certain neurons, we hope to make a "remote control" worm, which can be instructed to move either forward or backward by exposing it to the appropriate wavelengths of light.<ins class="diffchange diffchange-inline"><html></div></html></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Footnotes and Citations</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Footnotes and Citations</h2></div></td></tr>
</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=125010&oldid=prevGlh at 14:32, 23 October 20102010-10-23T14:32:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By combining these proteins in a single transgenic organism, behind specifically-chosen promoters which target only certain neurons, we hope to make a "remote control" worm, which can be instructed to move either forward or backward by exposing it to the appropriate wavelengths of light.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>By combining these proteins in a single transgenic organism, behind specifically-chosen promoters which target only certain neurons, we hope to make a "remote control" worm, which can be instructed to move either forward or backward by exposing it to the appropriate wavelengths of light.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Footnotes and Citations</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Footnotes and Citations</h2></div></td></tr>
</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=125008&oldid=prevGlh at 14:31, 23 October 20102010-10-23T14:31:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/head}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/head}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>Objective<del class="diffchange diffchange-inline">=</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h2></ins>Objective<ins class="diffchange diffchange-inline"></h2></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our main goal for this summer is to build a toolkit for the nematode ''Caenorhabditis elegans'' for use as a synthetic biology chassis. Our focus is on finding and selecting viable promoters, protein domains, and other components for use by future teams.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our main goal for this summer is to build a toolkit for the nematode ''Caenorhabditis elegans'' for use as a synthetic biology chassis. Our focus is on finding and selecting viable promoters, protein domains, and other components for use by future teams.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The main focus of our project is encouraging other teams to embrace the potential benefits of working in a complicated organism like ''C. elegans'', which has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, it represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The main focus of our project is encouraging other teams to embrace the potential benefits of working in a complicated organism like ''C. elegans'', which has been extensively studied as a model organism for developmental biology and was the first multicellular organism sequenced. As such, it represents one of the best opportunities for iGEM-style synthetic biology to make inroads into higher forms of life.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>R.''C. elegans''<del class="diffchange diffchange-inline">=</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h2></ins>R.''C. elegans''<ins class="diffchange diffchange-inline"></h2></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One diagnostic tool which has received a lot of attention recently are the '''channelrhodopsins''', which were isolated from the green algae ''Chlamydomonas reinhardtii'', and serve as light-activated nonspecific cation channels which can cause nerve cells to depolarise in response to certain wavelengths of light. These have been used extensively in research biology to interrogate the roles of the neurons in ''C. elegans'' and other species, allowing researchers to, for example, cause nematodes to contract all of their muscles at once and seize up in response to specific wavelengths of light.{{:Team:Queens-Canada/footnote-anchor|1}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>One diagnostic tool which has received a lot of attention recently are the '''channelrhodopsins''', which were isolated from the green algae ''Chlamydomonas reinhardtii'', and serve as light-activated nonspecific cation channels which can cause nerve cells to depolarise in response to certain wavelengths of light. These have been used extensively in research biology to interrogate the roles of the neurons in ''C. elegans'' and other species, allowing researchers to, for example, cause nematodes to contract all of their muscles at once and seize up in response to specific wavelengths of light.{{:Team:Queens-Canada/footnote-anchor|1}}</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>Footnotes and Citations<del class="diffchange diffchange-inline">=</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><h2></ins>Footnotes and Citations<ins class="diffchange diffchange-inline"></h2></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel]. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel]. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td></tr>
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</table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=121434&oldid=prevGlh: Team:Queens-Canada/Project moved to Team:Queens-Canada/idea2010-10-22T20:30:26Z<p><a href="/Team:Queens-Canada/Project" class="mw-redirect" title="Team:Queens-Canada/Project">Team:Queens-Canada/Project</a> moved to <a href="/Team:Queens-Canada/idea" title="Team:Queens-Canada/idea">Team:Queens-Canada/idea</a></p>
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</tr></table>Glhhttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=34770&oldid=prevThaiPhi at 19:39, 27 July 20102010-07-27T19:39:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Footnotes and Citations=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Footnotes and Citations=</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel]. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|Nagel G et al., 2003 [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel]. PNAS 100(24). doi:10.1073/pnas.1936192100}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|2|Han X, Boyden ES, 2007 [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299 Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution]. PLoS ONE 2(3): e299. doi:10.1371/journal.pone.0000299}} </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|2|Han X, Boyden ES, 2007 [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299 Multiple-Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution]. PLoS ONE 2(3): e299. doi:10.1371/journal.pone.0000299}} </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td></tr>
</table>ThaiPhihttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/idea&diff=34769&oldid=prevThaiPhi at 19:39, 27 July 20102010-07-27T19:39:10Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Footnotes and Citations=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Footnotes and Citations=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel]. PNAS. <del class="diffchange diffchange-inline">Accessed on 2010</del>-<del class="diffchange diffchange-inline">07</del>-<del class="diffchange diffchange-inline">27</del>.}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/footnote|1|<ins class="diffchange diffchange-inline">Nagel G et al., 2003 </ins>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC283525/ Channelrhodopsin-2, a directly light-gated cation-selective membrane channel]. PNAS <ins class="diffchange diffchange-inline">100(24)</ins>. <ins class="diffchange diffchange-inline">doi:10.1073/pnas.1936192100}}</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{:Team:Queens</ins>-<ins class="diffchange diffchange-inline">Canada/footnote|2|Han X, Boyden ES, 2007 [http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000299 Multiple</ins>-<ins class="diffchange diffchange-inline">Color Optical Activation, Silencing, and Desynchronization of Neural Activity, with Single-Spike Temporal Resolution]. PLoS ONE 2(3): e299. doi:10.1371/journal.pone</ins>.<ins class="diffchange diffchange-inline">0000299</ins>}} <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
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</table>ThaiPhi