Team:Queens-Canada/9 August 2010

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(Difference between revisions)
(New page: <h2>August 9, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Hao, Thai, Nelson, Chris, Yuli '''eCFP'''::1C3, '''NpHR'''::1C3, '''pStr-...)
 
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<h2>August 9, 2010</h2>
<h2>August 9, 2010</h2>
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Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V.
Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao
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Practiced injections with Sur-5::GFP marker.
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Latest revision as of 02:00, 28 October 2010

August 9, 2010

Miniprep

Hao, Thai, Nelson, Chris, Yuli

'eCFP::1C3, NpHR::1C3, pStr-1::1C3, pStr-220::1C3, pCeh-12::1C3, pOsm-10::1C3, pOdr-1::1C3, NpHR::1C3, pSrb-ChR2'::1C3, mCherry::1C3, pSB1C3


'pIC57ChR2'::1C3 did not pull through vacuum very well, may contain genomic DNA.

Gel and Digest

Chris and Thai

pOsm-10, pStr-220, pOdr-1

Cut with EcoRI and PstI.

Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V. Note: [4] pOsm-10 digest had <10 µL of liquid, most likely due to error during master mix loading.

Gel Electrophoresis

Chris and Thai

pOdr-1, NpHR, pStr-1, pCeh-12

Used 0.7 g agarose in 100 mL TBE. Gel run for 33 mins at 110 V.

Gel Electrophoresis

Chris and Thai

pCeh-12, pSrb-6, pIC576, eCFP

Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V.

Microinjection

Hao

Practiced injections with Sur-5::GFP marker.