Team:Queens-Canada/8 July 2010

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(Difference between revisions)
(New page: <h2>July 8, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Thai '''pGpd-2''', '''pFlp-1''' Program run using HIFI with an extension time of 25 s...)
 
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Cut using EcoRI and PstI.
Cut using EcoRI and PstI.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao and Yuli
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Practiced injections with water.
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Latest revision as of 01:13, 28 October 2010

July 8, 2010

PCR

Thai

pGpd-2, pFlp-1

Program run using HIFI with an extension time of 25 s and annealing temperature of 57 °C.

Heat Shock

Chris

pBluescriptII

Plates stored in 37 °C room overnight.

Gel Electrophoresis

Thai and Yuli

Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Hao and Chris

Used 0.4 g agarose in 60 mL TBE. Gel run for 45 mins at 100 V.

Digestion

Yuli

BBa_I13522

Cut using EcoRI and PstI.

Microinjection

Hao and Yuli

Practiced injections with water.