Team:Queens-Canada/7 October 2010

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(New page: <h2>October 7, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion-Ligation</h3> Thai and Hao '''eCFP'''::'''unc-54 3'UTR''', '''pOdr...)
 
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<h2>October 7, 2010</h2>
<h2>October 7, 2010</h2>
{{:Team:Queens-Canada/head}}
{{:Team:Queens-Canada/head}}
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Backbone treated with CIAP for 30 mins.
Backbone treated with CIAP for 30 mins.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao
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'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pHsp-3'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pFlp-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3
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Co-injected each construct with pRF4 marker.
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Latest revision as of 03:56, 28 October 2010

October 7, 2010

Digestion-Ligation

Thai and Hao

eCFP::unc-54 3'UTR, pOdr-10::eCFP::unc-54 3'UTR, pGpd-2::eCFP::unc-54 3'UTR, pSra-10::eCFP::unc-54 3'UTR, pFlp-1::eCFP::unc-54 3'UTR, ChR2::eCFP::unc-54 3'UTR, pHsp-3::eCFP::unc-54 3'UTR, pOsm-10::eCFP::unc-54 3'UTR, eYFP::eCFP::unc-54 3'UTR, mCherry::eCFP::unc-54 3'UTR, pStr-220::eCFP::unc-54 3'UTR, pSip-1::eCFP::unc-54 3'UTR, pOdr-1::eCFP::unc-54 3'UTR, pStr-1::eCFP::unc-54 3'UTR, pSB1C3

Backbone treated with CIAP for 30 mins.

Microinjection

Hao

pStr-1::eCFP::unc-54 3'UTR::1A3, pHsp-3::eCFP::unc-54 3'UTR::1A3, pFlp-1::eCFP::unc-54 3'UTR::1A3

Co-injected each construct with pRF4 marker.