Team:Queens-Canada/6 August 2010
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(New page: <h2>August 6, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Hao '''pSra-10''', '''pHsp-3''' Used 0.3 g agarose...) |
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<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | <h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | ||
Hao and Thai | Hao and Thai | ||
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'''ChR2''', '''eCFP''', '''eYFP''', '''mCherry''' | '''ChR2''', '''eCFP''', '''eYFP''', '''mCherry''' | ||
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<h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3> | <h3>[[Team:Queens-Canada/protocols#Heat Shock|Heat Shock]]</h3> | ||
Hao and Thai | Hao and Thai | ||
- | Ligation products from [[Team:Queens-Canada/ | + | Ligation products from [[Team:Queens-Canada/6 August 2010|06/08/10]] |
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> |
Revision as of 06:04, 27 October 2010
August 6, 2010
Gel Electrophoresis
Hao
pSra-10, pHsp-3
Used 0.3 g agarose in 40 mL TBE. Gel run for 50 mins at 100 V.
PCR
Hao and Thai
ChR2, eCFP, eYFP, mCherry
Program run using 9:1 FAST:HIFI blend with an extension time of 15 s and annealing temperature of 61 °C.
mCherry was run for 5 cycles without primers, then for 35 cycles with primers.
Ligation
Hao and Steve and Thai
NpHR, pOdr-10, eYFP, pStr-220, mCherry, pOsm-10, pOdr-1, eCFP, pMec-7, pFlp-1, pCeh-12, pIC518, ChR2, pSrb-6, pSB1C3
All inserts ligated into pSB1C3. T4 ligase used.
Heat Shock
Hao and Thai Ligation products from 06/08/10
Gel Electrophoresis
Hao and Thai
Used 0.3 g agarose in 45 mL TBE. Gel run for 45 mins at 100 V.