http://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/5_August_2010&feed=atom&action=historyTeam:Queens-Canada/5 August 2010 - Revision history2024-03-28T18:59:23ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/5_August_2010&diff=204585&oldid=prevHaoShi at 01:59, 28 October 20102010-10-28T01:59:26Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:59, 28 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 28:</td>
<td colspan="2" class="diff-lineno">Line 28:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Hao</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Practiced injections with Sur-5::GFP marker. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td></tr>
</table>HaoShihttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/5_August_2010&diff=203838&oldid=prevThaiPhi at 01:17, 28 October 20102010-10-28T01:17:56Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:17, 28 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 1:</td>
<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>August 5, 2010</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>August 5, 2010</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/head}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/head}}</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 11:</td>
<td colspan="2" class="diff-lineno">Line 10:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Hao and Basia and Thai</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Hao and Basia and Thai</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''pStr-1''', '''pMec-7''', '''pStr-1::1A2''', '''pSra-10''', '''pHsp-3'''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.</div></td></tr>
</table>ThaiPhihttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/5_August_2010&diff=174812&oldid=prevThaiPhi: New page: <h2>August 5, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Thai and Basia '''pMec-7''', '''pStr-1''' Program run using 9:1 FAST:HIFI blend wi...2010-10-27T06:02:46Z<p>New page: <h2>August 5, 2010</h2> {{:Team:Queens-Canada/head}} <h3><a href="/Team:Queens-Canada/protocols#PCR" title="Team:Queens-Canada/protocols">PCR</a></h3> Thai and Basia '''pMec-7''', '''pStr-1''' Program run using 9:1 FAST:HIFI blend wi...</p>
<p><b>New page</b></p><div><br />
<h2>August 5, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Thai and Basia<br />
<br />
'''pMec-7''', '''pStr-1'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C. <br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Basia and Thai<br />
<br />
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Hao and Chris<br />
<br />
'''pRab-7''', '''pSip-1''', '''pStr-1''', '''pHsp-3'''<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 110 V.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Chris<br />
<br />
'''pSra-10''', '''pHsp-3'''<br />
<br />
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>ThaiPhi