Team:Queens-Canada/5 August 2010

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(Difference between revisions)
(New page: <h2>August 5, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Thai and Basia '''pMec-7''', '''pStr-1''' Program run using 9:1 FAST:HIFI blend wi...)
 
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<h2>August 5, 2010</h2>
<h2>August 5, 2010</h2>
{{:Team:Queens-Canada/head}}
{{:Team:Queens-Canada/head}}
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
Hao and Basia and Thai
Hao and Basia and Thai
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'''pStr-1''', '''pMec-7''', '''pStr-1::1A2''', '''pSra-10''', '''pHsp-3'''
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.
Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.
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Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.
Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao
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Practiced injections with Sur-5::GFP marker.
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Latest revision as of 01:59, 28 October 2010

August 5, 2010

PCR

Thai and Basia

pMec-7, pStr-1

Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.

Gel Electrophoresis

Hao and Basia and Thai

pStr-1, pMec-7, pStr-1::1A2, pSra-10, pHsp-3

Used 0.7 g agarose in 100 mL TBE. Gel run for 60 mins at 100 V.

Gel Electrophoresis

Hao and Chris

pRab-7, pSip-1, pStr-1, pHsp-3

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 110 V.

PCR

Chris

pSra-10, pHsp-3

Program run using 9:1 FAST:HIFI blend with an extension time of 18 s and annealing temperature of 55 °C.

Microinjection

Hao

Practiced injections with Sur-5::GFP marker.