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Team:Queens-Canada/4 August 2010
From 2010.igem.org
(New page: <h2>August 4, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Basia and Thai Used 0.3 g agarose in 45 mL TBE. Gel...) |
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<h2>August 4, 2010</h2> | <h2>August 4, 2010</h2> | ||
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Hao and Basia | Hao and Basia | ||
| - | Re-performed heat shock from | + | Re-performed heat shock from [[Team:Queens-Canada/3 August 2010|03/08/10]] with leftover TOP10 cells and additional 5 µL of ligation product. Prepared new LB plates with Chlor antibiotics. |
Before plating, tubes were spun down at 13000g for 2 mins, the supernatant was discarded, and pellets were resuspended in 200µL of 2XTY. | Before plating, tubes were spun down at 13000g for 2 mins, the supernatant was discarded, and pellets were resuspended in 200µL of 2XTY. | ||
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Thai and Mary | Thai and Mary | ||
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| + | '''pStr-1::1A2''', '''unc-54 3'UTR::1A3''', '''pRab-7::1A3''' | ||
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 110 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 110 V. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 01:19, 28 October 2010
August 4, 2010
Gel Electrophoresis
Basia and Thai
Used 0.3 g agarose in 45 mL TBE. Gel run for 40 mins at 100 V.
Heat Shock
Hao and Basia
Re-performed heat shock from 03/08/10 with leftover TOP10 cells and additional 5 µL of ligation product. Prepared new LB plates with Chlor antibiotics. Before plating, tubes were spun down at 13000g for 2 mins, the supernatant was discarded, and pellets were resuspended in 200µL of 2XTY.
Miniprep
Hao and Thai
pStr-1::1A2, unc-54 3'UTR::1A3, pRab-7::1A3
HB Buffer was not used (not crucial).
PCR
Thai and Hao
pHsp-3, pSra-10
Program run using 9:1 FAST:HIFI blend with an extension time of 33 s and annealing temperature of 59 °C.
Digestion
Thai and Mary
Cut with EcoRI and PstI.
Gel Electrophoresis
Thai and Mary
pStr-1::1A2, unc-54 3'UTR::1A3, pRab-7::1A3
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 110 V.
