Team:Queens-Canada/31 August 2010

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(New page: <h2>August 31, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Hao and Thai '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pSra-...)
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
Hao and Chris
Hao and Chris
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PRF4
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'''PRF4'''
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.
Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.
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'''eGFP'''::'''unc-54 3'UTR'''::1A3, '''eYFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1A3
'''eGFP'''::'''unc-54 3'UTR'''::1A3, '''eYFP'''::'''unc-54 3'UTR'''::1A3, '''ChR2'''::'''unc-54 3'UTR'''::1A3
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{|
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{| border = "1"
| EcoRI and SpeI
| EcoRI and SpeI
| XbaI and PstI
| XbaI and PstI

Latest revision as of 01:11, 28 October 2010

August 31, 2010

Miniprep

Hao and Thai

pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3

Digestion

Hao and Thai

pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3

Gel Electrophoresis

Hao and Thai

pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3

Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.

Digestion

Hao and Chris PRF4, eYFP, eGFP

Gel Electrophoresis

Hao and Chris PRF4

Used 0.3 g agarose in 40 mL TBE. Gel run for 40 mins at 100 V.

Ligation

Hao and Chris

eGFP::unc-54 3'UTR::1A3, eYFP::unc-54 3'UTR::1A3, ChR2::unc-54 3'UTR::1A3

EcoRI and SpeI XbaI and PstI EcoRI and PstI
eYFP unc-54 3'UTR 1A3
eGFP unc-54 3'UTR 1A3
mCherry unc-54 3'UTR 1A3

Backbone treated with CIAP for 30 mins.

Heat Shock

Hao and Chris

eGFP::unc-54 3'UTR::1A3, eYFP::unc-54 3'UTR::1A3, mCherry::unc-54 3'UTR::1A3