Team:Queens-Canada/30 August 2010

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(New page: <h2>August 30, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Thai '''pOsm-10'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''...)
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<h2>August 30, 2010</h2>
<h2>August 30, 2010</h2>
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Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]]
Transformed ligation products from [[Team:Queens-Canada/30 August 2010|30/08/10]]
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao
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'''pMec-7'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3
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Transferred F1 transgenic worms to new plate
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Revision as of 01:00, 28 October 2010

August 30, 2010

Liquid Culture

Thai

pOsm-10::eCFP::unc-54 3'UTR::1A3, pSra-10::eCFP::unc-54 3'UTR::1A3

Digestion

Hao and Thai

pSip-1::1C3, pFlp-1::1C3, pOdr-10::1C3, mCherry::1C3

EcoRI and SpeI XbaI and PstI EcoRI and PstI
pStr-220::1C3 eCFP::unc-54 3'UTR::1T3 pSB1T3
mCherry::1C3 unc-54 3'UTR::1C3
pFlp-1::1C3
pSip-1::1C3
pOdr-10::1C3

Backbone treated with CIAP for 30 mins.

Ligation

Hao and Thai

pSip-1, pStr-220, pFlp-1, pOdr-10::eCFP::unc-54 3'UTR::1A3, ChR2::unc-54 3'UTR::1T3

EcoRI and SpeI XbaI and PstI EcoRI and PstI
pStr-1 eCFP::unc-54 3'UTR 1A3
pStr-220 eCFP::unc-54 3'UTR 1A3
pFlp-1 eCFP::unc-54 3'UTR 1A3
pOdr-10 eCFP::unc-54 3'UTR 1A3
mCherry unc-54 3'UTR 1T3

Backbone treated with CIAP for 30 mins.

Heat Shock

Hao

pSip-1, pStr-220, pFlp-1, pOdr-10::eCFP::unc-54 3'UTR::1A3, ChR2::unc-54 3'UTR::1T3

Transformed ligation products from 30/08/10

Microinjection

Hao

pMec-7::eCFP::unc-54 3'UTR::1C3

Transferred F1 transgenic worms to new plate