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Team:Queens-Canada/2 September 2010
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(New page: <h2>September 2, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Miniprep</h3> Steve and Chris '''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR...) |
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<h2>September 2, 2010</h2> | <h2>September 2, 2010</h2> | ||
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'''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3 | '''eYFP''', '''eGFP''', '''mCherry'''::'''unc-54 3'UTR'''::1A3 | ||
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| + | <h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3> | ||
| + | Hao | ||
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| + | No transgenic F1 from 02/09/10 injections. | ||
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| + | '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3, '''pOdr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1A3 | ||
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| + | Co-injected each construct with Sur-5::GFP marker. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 02:27, 28 October 2010
September 2, 2010
Miniprep
Steve and Chris
eYFP, eGFP, mCherry::unc-54 3'UTR::1A3
Digestion
Steve and Chris
eYFP, eGFP, mCherry::unc-54 3'UTR::1A3
Gel Electrophoresis
Chris and Steve
eYFP, eGFP, mCherry::unc-54 3'UTR::1A3
Microinjection
Hao
No transgenic F1 from 02/09/10 injections.
pStr-1::eCFP::unc-54 3'UTR::1A3, pOdr-1::eCFP::unc-54 3'UTR::1A3
Co-injected each construct with Sur-5::GFP marker.
