http://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/28_June_2010&feed=atom&action=historyTeam:Queens-Canada/28 June 2010 - Revision history2024-03-28T16:22:01ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/28_June_2010&diff=203595&oldid=prevThaiPhi at 01:11, 28 October 20102010-10-28T01:11:12Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:11, 28 October 2010</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 18:</td>
<td colspan="2" class="diff-lineno">Line 18:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Hao and Yuli</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Practiced injections with water.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:Queens-Canada/foot}}</div></td></tr>
</table>ThaiPhihttp://2010.igem.org/wiki/index.php?title=Team:Queens-Canada/28_June_2010&diff=174464&oldid=prevThaiPhi: New page: <h2>June 28, 2010</h2> {{:Team:Queens-Canada/head}} <h3>PCR</h3> Steve '''pSip-1''', '''pMec-7''', '''pRab-7''' For '''pSip-1''' and '''pRab-7''': Pr...2010-10-27T05:37:14Z<p>New page: <h2>June 28, 2010</h2> {{:Team:Queens-Canada/head}} <h3><a href="/Team:Queens-Canada/protocols#PCR" title="Team:Queens-Canada/protocols">PCR</a></h3> Steve '''pSip-1''', '''pMec-7''', '''pRab-7''' For '''pSip-1''' and '''pRab-7''': Pr...</p>
<p><b>New page</b></p><div><h2>June 28, 2010</h2><br />
{{:Team:Queens-Canada/head}}<br />
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3><br />
Steve<br />
<br />
'''pSip-1''', '''pMec-7''', '''pRab-7'''<br />
<br />
For '''pSip-1''' and '''pRab-7''':<br />
Program run using HIFI with an extension time of 12 s and annealing temperature of 58 °C.<br />
<br />
For '''pMec-7''':<br />
Program run using HIFI with an extension time of 30 s and annealing temperature of 56 °C.<br />
<br />
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3><br />
Basia and Yuli<br />
<br />
'''pSip-1''':::'''eGFP''' Extract, '''pRab-7''':::'''eGFP''' Extract, '''pMec-7''':::'''eGFP''' Extract, '''unc-54 3'UTR'''<br />
<br />
Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.<br />
<br />
{{:Team:Queens-Canada/foot}}</div>ThaiPhi