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Team:Queens-Canada/22 June 2010
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(New page: <h2>June 22, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Yuli and Hao '''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-...)
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(New page: <h2>June 22, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Digestion</h3> Yuli and Hao '''pSip-1'''::'''eGFP''', '''pRab-7'''::'''eGFP''', '''pMec-...)
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Revision as of 05:33, 27 October 2010
June 22, 2010
Digestion
Yuli and Hao
pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP, unc-54 3'UTR
Cut using XbaI and SpeI. Incubated at 37 °C for 1 hour. Enzymes inactivated at 80 ° C for 20 mins.
Ligation
Hao and Yuli
pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP, unc-54 3'UTR
Ligated using T4 ligase.
Gel Electrophoresis
Hao and Thai 3'UTR digestion & ligation products
