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Team:Queens-Canada/22 July 2010
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(Difference between revisions)
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<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3> | <h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3> | ||
Hao and Thai | Hao and Thai | ||
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<h3>[[Team:Queens-Canada/protocols#Sequencing Prep|Sequencing Prep]]</h3> | <h3>[[Team:Queens-Canada/protocols#Sequencing Prep|Sequencing Prep]]</h3> | ||
Latest revision as of 05:55, 27 October 2010
July 22, 2010
Gel Electrophoresis
Steve
eYFP Extract
Used 0.7 g agarose in 100 mL TBE. Gel run for 50 mins at 100 V.
Miniprep
Hao and Thai
PRF4
Sequencing Prep
Chris and Thai
pSip-1::1A3
Used 4 µL of 2µM primers with 8 µL of plasmid.
PCR
Yuli
pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR, pStr-1
Program run using 9:1 FAST:HIFI blend with an extension time of 45 s and annealing temperature of 61 °C.
Gel Electrophoresis
Chris
pSip-1:::eGFP, pRab-7:::eGFP, pMec-7:::eGFP, unc-54 3'UTR, pStr-1
Used 0.3 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.
PCR
Steve
pSB1A3
Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 62 °C.
PCR
Yuli
eYFP1a and eYFP2a
Program run using 9:1 FAST:HIFI blend with an extension time of 45 s and annealing temperature of 58 °C.
