Team:Queens-Canada/19 July 2010

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(New page: <h2>July 19, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Gel Electrophoresis</h3> Yuli Used 0.7 g agarose in 100 mL TBE. Gel run for 4...)
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<h2>July 19, 2010</h2>
<h2>July 19, 2010</h2>
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Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V.
Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao
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Practiced injections with water
{{:Team:Queens-Canada/foot}}
{{:Team:Queens-Canada/foot}}

Revision as of 01:50, 28 October 2010

July 19, 2010

Gel Electrophoresis

Yuli

Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Hao

pSra-10

Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.

Liquid Culture

Hao

pSip-1::1A3 (Amp), BBa_I13522

Diagnostic Digestion

Yuli

pSip-1::1A3

Cut with: XbaI, SpeI, and PstI or NotI, EcoRI.

Gel Electrophoresis

Yuli and Chris

pSip-1 Digests

Used 0.3 g agarose in in 50 mL TBE. Gel run for 45 mins at 100 V.

Microinjection

Hao

Practiced injections with water