Team:Queens-Canada/18 June 2010

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[[Team:Queens-Canada/29 June 2010]]
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{{:Team:Queens-Canada/head}}
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<h2>June 18, 2010</h2>
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<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3>
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Hao and Yuli
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'''pSip-1''', '''pHsp-3''', '''pUnc-47''', '''pRab-7''', '''pMec-7''' with '''BBa_I714891'''
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Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles.
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Primers were then added and program was run with annealing temperature of 1 min 15 s.
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
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Hao and Yuli
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'''pSip-1'''::'''eGFP''', '''pHsp-3'''::'''eGFP''', '''pMec-7'''::'''eGFP''', '''pUnc-47'''::'''eGFP''', '''pRab-7'''::'''eGFP'''
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Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.
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<h3>[[Team:Queens-Canada/transformation|Microinjection]]</h3>
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Hao and Yuli
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Practiced injections with water
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{{:Team:Queens-Canada/foot}}

Latest revision as of 01:06, 28 October 2010

June 18, 2010

PCR

Hao and Yuli

pSip-1, pHsp-3, pUnc-47, pRab-7, pMec-7 with BBa_I714891

Program run using HIFI with an extension time of 3 min and annealing temperature of 57 °C without primers for 5 cycles. Primers were then added and program was run with annealing temperature of 1 min 15 s.

Gel Electrophoresis

Hao and Yuli

pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP

Used 0.7 g agarose in 100 mL TBE. Gel run for 45 mins at 100 V.

Microinjection

Hao and Yuli

Practiced injections with water