"
Team:Queens-Canada/17 June 2010
From 2010.igem.org
(Difference between revisions)
| Line 17: | Line 17: | ||
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | <h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3> | ||
Yuli | Yuli | ||
| + | |||
PCR Purification products from June 16, 2010 | PCR Purification products from June 16, 2010 | ||
Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V. | Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V. | ||
Latest revision as of 05:23, 27 October 2010
Contents |
June 17, 2010
Gel Electrophoresis
Kevin and Nelson
pSip-1::eGFP, pHsp-3::eGFP, pMec-7::eGFP, pUnc-47::eGFP, pRab-7::eGFP
Used 1.1 g agarose in 160 mL TBE. Gel run for 40 mins at 100 V.
Digestion
Nelson
BBa_J45200, BBa_J45120
Incubated at 37 °C for 20 mins. Products stored at -20 °C.
Gel Electrophoresis
Yuli
PCR Purification products from June 16, 2010
Used 0.4 g agarose in in 60 mL TBE. Gel run for 40 mins at 100 V.
