Team:Queens-Canada/16 June 2010

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==June 16, 2010==
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{{:Team:Queens-Canada/head}}
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====[[Team:Queens-Canada/protocols#PCR Purification|PCR Purification]]====
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<h2>June 16, 2010</h2>
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<h3>[[Team:Queens-Canada/protocols#PCR Purification|PCR Purification]]</h3>
Hao and Chris
Hao and Chris
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Products stored in -20 °C freezer.
Products stored in -20 °C freezer.
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====[[Team:Queens-Canada/protocols#PCR|PCR]]====
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<h3>[[Team:Queens-Canada/protocols#PCR Stitching|PCR Stitching]]</h3>
Hao and Chris
Hao and Chris
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Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles.
Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles.
Primers were then added and program was run with annealing temperature of 60 °C.
Primers were then added and program was run with annealing temperature of 60 °C.
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{{:Team:Queens-Canada/foot}}

Latest revision as of 05:21, 27 October 2010

June 16, 2010

PCR Purification

Hao and Chris

pHsp-3, pSip-1, pMec-7, pRab-7, pUnc-47, BBa_I714891

Products stored in -20 °C freezer.

PCR Stitching

Hao and Chris

pHsp-3, pRab-7, pSip-1, pUnc-47, pMec-7 with BBa_I714891

Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles. Primers were then added and program was run with annealing temperature of 60 °C.