Team:Queens-Canada/16 June 2010


(Difference between revisions)
(New page: ==June 16, 2010== ====PCR Purification==== Hao and Chris '''pHsp-3''', '''pSip-1''', '''pMec-7''', '''pRab-7''', '''pUnc-47''', '''BBa_I7...)
(PCR Stitching)
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Products stored in -20 °C freezer.
Products stored in -20 °C freezer.
====[[Team:Queens-Canada/protocols#PCR Stitching|PCR Stitching]]====
Hao and Chris
Hao and Chris

Revision as of 05:05, 27 October 2010

June 16, 2010

PCR Purification

Hao and Chris

pHsp-3, pSip-1, pMec-7, pRab-7, pUnc-47, BBa_I714891

Products stored in -20 °C freezer.


Hao and Chris

pHsp-3, pRab-7, pSip-1, pUnc-47, pMec-7 with BBa_I714891

Program run (without primers) using TAQ with an extension time of 30 s and annealing temperature of 57 °C for 5 cycles. Primers were then added and program was run with annealing temperature of 60 °C.