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Team:Queens-Canada/16 July 2010
From 2010.igem.org
July 16, 2010
PCR
Yuli and Thai
eYFP1a
Program run using HIFI with an extension time of 30 s and annealing temperature of 61 °C.
Digestion
Yuli
pSip-1::1A3
Cut with XbaI and PstI.
Gel Electrophoresis
Thai and Yuli
Used 0.3 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.
