Team:Queens-Canada/16 July 2010

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July 16, 2010

PCR

Yuli and Thai

eYFP1a

Program run using HIFI with an extension time of 30 s and annealing temperature of 61 °C.

Digestion

Yuli

pSip-1::1A3

Cut with XbaI and PstI.

Gel Electrophoresis

Thai and Yuli

Used 0.3 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V.

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