Team:Queens-Canada/16 August 2010

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August 16, 2010

PCR

Yuli

pMec-7::eGFP

Program run using 9:1 FAST:HIFI blend with an extension time of 20 s and annealing temperature of 60 °C.

Miniprep

Thai and Chris Culture tubes from 13/08/10

Gel Electrophoresis

Thai

Used 0.3 g agarose in 40 mL TBE. Gel run for 45 mins at 100 V.

Gel Electrophoresis

Yuli

pSip-1, pRab-7, mCherry, pFlp-1

Gel Electrophoresis

Thai and Yuli

pCeh-12::1C3, ChR2::eYFP::1C3, eGFP, pSB1C3, ChR2::eYFP::unc-54 3'UTR::1T3

Used 0.7 g agarose in 100 mL TBE. Gel run for 30 mins at 100 V.