Team:Queens-Canada/15 June 2010

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Contents

June 15, 2010

Liquid Culture

Thai and Chris

BBa_J45200, BBa_J45120

Products incubated in 37°C room on shaker.

PCR

Yuli

BBa_I714891

Program run using TAQ with extension time of 30s and annealing temperature of 51°C.

Glycerol Stock

Hao and Mary

BBa_J45200, BBa_J45120

Change: Used 250 µL of 80% glycerol and 750 µL of overnight culture. Stored in -80 °C freezer.

Miniprep

Hao and Mary

BBa_J45200, BBa_J45120

Standard procedure followed.