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Team:Queens-Canada/15 June 2010
From 2010.igem.org
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<h2>June 15, 2010</h2> | <h2>June 15, 2010</h2> | ||
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3> | <h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3> | ||
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Standard procedure followed. | Standard procedure followed. | ||
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Latest revision as of 05:21, 27 October 2010
June 15, 2010
Liquid Culture
Thai and Chris
BBa_J45200, BBa_J45120
Products incubated in 37°C room on shaker.
PCR
Yuli
BBa_I714891
Program run using TAQ with extension time of 30s and annealing temperature of 51°C.
Glycerol Stock
Hao and Mary
BBa_J45200, BBa_J45120
Change: Used 250 µL of 80% glycerol and 750 µL of overnight culture. Stored in -80 °C freezer.
Miniprep
Hao and Mary
BBa_J45200, BBa_J45120
Standard procedure followed.
