Team:Queens-Canada/15 June 2010

From 2010.igem.org

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==June 15, 2010==
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{{:Team:Queens-Canada/head}}
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====[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]====
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<h2>June 15, 2010</h2>
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<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3>
Thai and Chris
Thai and Chris
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Products incubated in 37°C room on shaker.
Products incubated in 37°C room on shaker.
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====[[Team:Queens-Canada/protocols#PCR|PCR]]====
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<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3>
Yuli
Yuli
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Program run using TAQ with extension time of 30s and annealing temperature of 51°C.
Program run using TAQ with extension time of 30s and annealing temperature of 51°C.
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====[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]====
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<h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3>
Hao and Mary
Hao and Mary
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Stored in -80 °C freezer.
Stored in -80 °C freezer.
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====[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]====
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<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3>
Hao and Mary
Hao and Mary
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Standard procedure followed.
Standard procedure followed.
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{{:Team:Queens-Canada/foot}}

Latest revision as of 05:21, 27 October 2010

June 15, 2010

Liquid Culture

Thai and Chris

BBa_J45200, BBa_J45120

Products incubated in 37°C room on shaker.

PCR

Yuli

BBa_I714891

Program run using TAQ with extension time of 30s and annealing temperature of 51°C.

Glycerol Stock

Hao and Mary

BBa_J45200, BBa_J45120

Change: Used 250 µL of 80% glycerol and 750 µL of overnight culture. Stored in -80 °C freezer.

Miniprep

Hao and Mary

BBa_J45200, BBa_J45120

Standard procedure followed.