Team:Queens-Canada/15 June 2010

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(Difference between revisions)
(New page: ==June 15, 2010== ====Liquid Culture==== Thai and Chris '''BBa_J45200''', '''BBa_J45120''' Products incubated in 37°C room on shaker. ====PCR==== Yuli '''BBa_I714891''' Program run u...)
 
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==June 15, 2010==
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{{:Team:Queens-Canada/head}}
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====Liquid Culture====
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<h2>June 15, 2010</h2>
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<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3>
Thai and Chris
Thai and Chris
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Products incubated in 37°C room on shaker.
Products incubated in 37°C room on shaker.
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====PCR====
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<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3>
Yuli
Yuli
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Program run using TAQ with extension time of 30s and annealing temperature of 51°C.
Program run using TAQ with extension time of 30s and annealing temperature of 51°C.
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====Glycerol Stock====
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<h3>[[Team:Queens-Canada/protocols#Glycerol Stock|Glycerol Stock]]</h3>
Hao and Mary
Hao and Mary
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Stored in -80 °C freezer.
Stored in -80 °C freezer.
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====Miniprep====
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<h3>[[Team:Queens-Canada/protocols#Miniprep|Miniprep]]</h3>
Hao and Mary
Hao and Mary
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Standard procedure followed.
Standard procedure followed.
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{{:Team:Queens-Canada/foot}}

Latest revision as of 05:21, 27 October 2010

June 15, 2010

Liquid Culture

Thai and Chris

BBa_J45200, BBa_J45120

Products incubated in 37°C room on shaker.

PCR

Yuli

BBa_I714891

Program run using TAQ with extension time of 30s and annealing temperature of 51°C.

Glycerol Stock

Hao and Mary

BBa_J45200, BBa_J45120

Change: Used 250 µL of 80% glycerol and 750 µL of overnight culture. Stored in -80 °C freezer.

Miniprep

Hao and Mary

BBa_J45200, BBa_J45120

Standard procedure followed.