Team:Queens-Canada/15 July 2010

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(Difference between revisions)
(New page: <h2>July 15, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Hao '''BBa_I13522''', '''pSip-'''::1A3 <h3>[[Team:Queens-Canad...)
 
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<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3>
<h3>[[Team:Queens-Canada/protocols#Digestion|Digestion]]</h3>
Yuli and Thai
Yuli and Thai
 +
'''pSB1A3'''::'''pSip-1'''
'''pSB1A3'''::'''pSip-1'''
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
Yuli
Yuli
 +
'''pSB1A3'''::'''pSip-1'''
'''pSB1A3'''::'''pSip-1'''

Latest revision as of 05:50, 27 October 2010

July 15, 2010

Liquid Culture

Hao

BBa_I13522, pSip-::1A3

Diagnostic PCR

Thai and Hao

pStr-1

Re-run blends A, D, H, K, L, and M from July 14th using an extension time of 15 s, and an annealing temperature of 55 °C.

Gel Electrophoresis

Geoff and Thai

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.

Heat Shock

Chris

pRF4

Plates stored in 37 °C room overnight.

Digestion

Yuli and Thai

pSB1A3::pSip-1

Cut with XbaI and PstI.

Gel Electrophoresis

Yuli

pSB1A3::pSip-1

Used 0.4 g agarose in 60 mL TBE. Gel run for 40 mins at 100 V.