Team:Queens-Canada/14 September 2010


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September 14, 2010


Steve and Yuli

ChR2::eYFP::unc-54 3'UTR::1C3

Cut with EcoRI and PstI.

Gel Electrophoresis

Steve and Nelson

ChR2::eYFP::unc-54 3'UTR::1C3, ChR2::eYFP::unc-54 3'UTR::1C3 Digest

Used 0.5 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.


Yuli and Nelson and Hao

NpHR::eCFP::1C3, eGFP::1C3, pSB1A3, ChR2::eYFP::1C3