Team:Queens-Canada/14 October 2010
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(New page: <h2>October 14, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Heat Shock</h3> Steve '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team...)
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(New page: <h2>October 14, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Heat Shock</h3> Steve '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team...)
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Revision as of 06:20, 27 October 2010
October 14, 2010
Heat Shock
Steve
pStr-1::eCFP::unc-54 3'UTR::1C3 from 07/10/10
Liquid Culture
Steve
pFlp-1::eCFP::unc-54 3'UTR::1C3, pSra-10::eCFP::unc-54 3'UTR::1C3, ChR2::eYFP::unc-54 3'UTR::1C3, pHsp-3::eCFP::unc-54 3'UTR::1C3, pOsm-10::eCFP::unc-54 3'UTR::1C3, pOdr-1::eCFP::unc-54 3'UTR::1C3, eYFP::unc-54 3'UTR::1C3, mCherry::unc-54 3'UTR::1C3
Digestion-Ligation
Steve
pGpd-2, pOdr-10, pStr-220, pOdr-10, pSip-1, eCFP::unc-54 3'UTR::1C3
Gel Electrophoresis
Steve Ligation products from 14/10/10
Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.