Team:Queens-Canada/14 October 2010

From 2010.igem.org

(Difference between revisions)
(New page: <h2>October 14, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Heat Shock</h3> Steve '''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team...)
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<h2>October 14, 2010</h2>
<h2>October 14, 2010</h2>
{{:Team:Queens-Canada/head}}
{{:Team:Queens-Canada/head}}
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Steve
Steve
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'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team:Queens-Canada/07 October 2010|07/10/10]]
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'''pStr-1'''::'''eCFP'''::'''unc-54 3'UTR'''::1C3 from [[Team:Queens-Canada/7 October 2010|07/10/10]]
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3>
<h3>[[Team:Queens-Canada/protocols#Liquid Culture|Liquid Culture]]</h3>
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<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
<h3>[[Team:Queens-Canada/protocols#Gel Electrophoresis|Gel Electrophoresis]]</h3>
Steve
Steve
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Ligation products from [[Team:Queens-Canada/14 October 2010|14/10/10]]
Ligation products from [[Team:Queens-Canada/14 October 2010|14/10/10]]

Revision as of 06:21, 27 October 2010

October 14, 2010

Heat Shock

Steve

pStr-1::eCFP::unc-54 3'UTR::1C3 from 07/10/10

Liquid Culture

Steve

pFlp-1::eCFP::unc-54 3'UTR::1C3, pSra-10::eCFP::unc-54 3'UTR::1C3, ChR2::eYFP::unc-54 3'UTR::1C3, pHsp-3::eCFP::unc-54 3'UTR::1C3, pOsm-10::eCFP::unc-54 3'UTR::1C3, pOdr-1::eCFP::unc-54 3'UTR::1C3, eYFP::unc-54 3'UTR::1C3, mCherry::unc-54 3'UTR::1C3

Digestion-Ligation

Steve

pGpd-2, pOdr-10, pStr-220, pOdr-10, pSip-1, eCFP::unc-54 3'UTR::1C3

Gel Electrophoresis

Steve

Ligation products from 14/10/10

Used 0.3 g agarose in 50 mL TBE. Gel run for 40 mins at 100 V.